Strain Improvement Through Protoplast Fusion for Enhanced Coffee Pulp Degradation
نویسندگان
چکیده
Pleurotus eous colonies regenerated on plates containing complete medium overlaid with soft agar gave 0.28% regeneration efficiency, while that of Pleurotus flabellatus was slightly less (0.24%). In PEG (polyethylene glycol) induced fusion experiments with Pleurotus eous and Pleurotus flabellatus, 0.18% of fusion frequency was achieved and four fusant colonies were recovered. The fusant No.4 could grow on regeneration minimal medium and minimal medium.
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